Imaging the actin cytoskeleton in cells uses a wide range of approaches. Typically, a fluorescent derivative of the small cyclic peptide phalloidin is used to image F-actin in fixed cells. Lifeact and F-tractin are popular for imaging the cytoskeleton in live cells.
Mass spectrometry‐based proteomics identifies putative substrates of the ubiquitin‐dependent remodeler VCP in human cells and reveals its role in the regulation of the c‐Myc level and activity.
A novel Affimer-functionalised interdigitated electrode-based capacitive biosensor platform was developed for detection and estimation of Her4, a protein tumour biomarker, in undiluted serum.
This review examines the potential of scaffold-based binding proteins to fulfil these requirements.
Damaged mitochondria undergo mitophagy, a specialized form of autophagy that is initiated by the protein kinase PINK1 and the ubiquitin E3 ligase Parkin.
Michel et al. describe linkage-specific tools for the study of atypical K6 and K33/ K11 ubiquitin linkages. Affimers have applications in western blotting, confocal microscopy, and pull-downs.
Glypican-3 (GPC3) is a promising new marker for hepatocellular carcinoma, but the reported values for serum GPC3 differ markedly between currently available kits.
Antibodies have been indispensable tools in molecular biology, biochemistry and medical research.
This paper reports on the use of Affimer proteins as research reagents. It shows that Affimer proteins represent complementary affinity reagents to antibodies for various molecular and cell biology applications.
An ultraefficient cap-exchange protocol (UCEP) that can convert hydrophobic quantum dots (QDs) into stable, biocompatible, and aggregation-free water-dispersed ones at a ligand:QD molar ratio (LQMR) as low as 500, some 20−200-fold less than most literature methods, has been developed.