Damaged mitochondria undergo mitophagy, a specialized form of autophagy that is initiated by the protein kinase PINK1 and the ubiquitin E3 ligase Parkin.
Michel et al. describe linkage-specific tools for the study of atypical K6 and K33/ K11 ubiquitin linkages. Affimers have applications in western blotting, confocal microscopy, and pull-downs.
Glypican-3 (GPC3) is a promising new marker for hepatocellular carcinoma, but the reported values for serum GPC3 differ markedly between currently available kits.
Antibodies have been indispensable tools in molecular biology, biochemistry and medical research.
This paper reports on the use of Affimer proteins as research reagents. It shows that Affimer proteins represent complementary affinity reagents to antibodies for various molecular and cell biology applications.
An ultraefficient cap-exchange protocol (UCEP) that can convert hydrophobic quantum dots (QDs) into stable, biocompatible, and aggregation-free water-dispersed ones at a ligand:QD molar ratio (LQMR) as low as 500, some 20−200-fold less than most literature methods, has been developed.
Conventional immunoassays rely on antibodies that provide high affinity, specificity, and selectivity against a target analyte.
Selection methods are used to identify Affimers that recognise α-helix mimicking
To enable multiplexed protein analysis through the use of microarrays, reliable molecules capable of specifically binding to a protein of interest with high affinity are required. Further, this specificity and affinity must be retained upon immobilization to the microarray surface.
Biosensors with high sensitivity and short time-to-result that are capable of detecting biomarkers in body fluids such as serum are an important prerequisite for early diagnostics in modern healthcare provision.