Anti-Trastuzumab Affimer Reagent – 6xHis tag, Cysteine

Catalogue CodeAVA00106 Size100μg
 
Highly specific Affimer® reagent against the humanised drug Trastuzumab (Herceptin).

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Anti-Trastuzumab Affimer Reagent – 6xHis tag, Cysteine
£200.00
 
Catalogue CodeAVA00106 Size100μg
 
Highly specific Affimer® reagent against the humanised drug Trastuzumab (Herceptin).
OR
Datasheet

Datasheet

General  
Name Anti-Trastuzumab Affimer Reagent – 6xHis tag, Cysteine
Catalogue Code AVA00106
Overview  
Clone ID 386_737_B3
Size (μg) 100
Tested Applications ELISA
Tags 6X His, Cys
Conjugate Unconjugated
Properties  
Form Liquid
Storage Instructions Store at 4oC for up to 6 months. For longer term storage, it is recommended to store at -20oC in single use aliquots.
Buffer 100mM Sodium Citrate, 150mM Sodium Chloride, 5mM TCEP, 0.02% Sodium Azide, pH 6.5
Purity >95%
Purification Method IMAC-SEC
Clonality Monoclonal
Target  
Target Trastuzumab (Herceptin)
Species Reactivity Human
Target Function Anti-trastuzumab Affimer binders show excellent sensitivity to the therapeutic antibody target comparable to the natural ligand, HER2. Capture ELISA data using anti-trastuzumab Affimer reagents passively adsorbed onto ELISA plate showed detection of the trastuzumab antibody target over the clinically relevant concentration range 30-1600 ng/mL, performing in line with the natural HER2 ligand, to a lower concentration of 30 ng/mL.
Research Area Toolkit
Applications

Applications

Direct ELISA

AVA00106 Direct ELISA with legend
Affimer Coating
Maxisorp plates coated with 100μL/well Anti-Trastuzumab Affimer 386_737_B3 (1μg/mL) in 1xCarbonate/Bicarbonate buffer, pH 9.6 (16 h, 4°C)
Blocking
Blocked 300μL/well in 1 x Casein Block (Sigma) in 1xPBS (2 h, RT, gently shaking)
Wash 1
3x 300μL/well of 1xPBS-T (0.05% Tween-20) using plate washer
Sample incubation:
Dilute sample 1/10 (MRD), 1 x Casein Block (Sigma) in 1xPBS (1 h, RT, gently shaking)
Wash 2
3x 300μL/well of 1xPBS-T (0.05% Tween-20) using plate washer
Detection
1 in 100,000 anti-human IgG Fc HRP (Bethyl) in 1 x Casein Block (Sigma) in 1xTBS (1 h, RT°C, roller)
Wash 3
3x 300μL/well of 1xPBS-T (0.05% Tween-20) using plate washer
Substrate
MB (Surmodics)
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