Anti-Ipilimumab Affimer Reagent – HA, 6xHis tag

Catalogue CodeAVA00109 Size100μg
 
Highly specific Affimer® reagent against the humanised drug Ipilimumab

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Anti-Ipilimumab Affimer Reagent – HA, 6xHis tag
£200.00
 
Catalogue CodeAVA00109 Size100μg
 
Highly specific Affimer® reagent against the humanised drug Ipilimumab
OR
Datasheet

Datasheet

General  
Name Anti-Ipilimumab Affimer Reagent – HA, 6xHis tag
Catalogue Code AVA00109
Overview  
Clone ID 433_638869
Size (μg) 100
Tested Applications ELISA
Tags HA, 6xHis
Conjugate Unconjugated
Properties  
Form Liquid
Storage Instructions Store at 4oC for up to 6 months. For longer term storage, it is recommended to store at -20oC in single use aliquots.
Buffer 100 mM Sodium Phosphate, 150 mM Sodium Chloride, 0.02% Sodium Azide, pH 7.4
Purity >95%
Purification Method IMAC-SEC
Clonality Monoclonal
Target  
Target Ipilimumab
Species Reactivity Human
Target Function Anti-Ipilimumab Affimer reagent shows excellent sensitivity to the therapeutic antibody target where performance is comparable to its target, CTLA-4. Capture ELISA data using Anti-Ipilimumab Affimer® reagent passively adsorbed onto ELISA plates showed detection of Ipilimumab antibody over the clinically relevant concentration range 15-1000 ng/mL.
Research Area Immunology
Applications

Applications

Direct ELISA

AVA00109 Direct ELISA
Coating
Maxisorp plates coated with 100 μL/well Anti-Humira Affimer® Reagent (1 μg/mL) in 1 x Carbonate/Bicarbonate buffer, pH 9.6 (16 h, 4°C)
Blocking
Blocked 300 μL/well in 1 x Casein Block (Sigma) in 1 x PBS (2 h, room temperature (RT))
Wash 1
3 x 300 μL/well of 1 x PBS-T (0.05% Tween-20)
Sample incubation
Prepare standard curve in 1 x Casein Block (Sigma) in 1 x PBS (1 h, RT)
Wash 2
3 x 300 μL/well of 1 x PBS-T (0.05% Tween-20)
Detection
1 in 100,000 anti-human IgG Fc HRP conjugated (Bethyl A80-319P) in 1 x Casein Block (Sigma) (1 h, RT)
Wash 3
3 x 300 μL/well of 1 x PBS-T (0.05% Tween-20)
Substrate
TMB (Surmodics)
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