Anti-diUbiquitin K6 Affimer Reagent – 6xHis tag, Cysteine

Catalogue CodeAVA00101 Size100μg
 
Anti di-Ubiquitin K6 Affimer® Reagent (with 6X His and Cys tags) is a unique binder with no antibody equivalent.

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Anti-diUbiquitin K6 Affimer Reagent – 6xHis tag, Cysteine
£200.00
 
Catalogue CodeAVA00101 Size100μg
 
Anti di-Ubiquitin K6 Affimer® Reagent (with 6X His and Cys tags) is a unique binder with no antibody equivalent.
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Datasheet

Datasheet

General  
Name Anti-diUbiquitin K6 Affimer Reagent – 6xHis tag, Cysteine
Catalogue Code AVA00101
Overview  
Clone ID K6-29
Size (μg) 100
Tested Applications Western Blotting
Tags 6X His; Cys
Conjugate Unconjugated
Properties  
Form Liquid
Storage Instructions Store at 4oC for up to 6 months. For longer term storage, it is recommended to store at -20oC in single use aliquots.
Buffer 100mM Sodium Citrate, 150mM Sodium Chloride, 5 mM TCEP, 0.02% Sodium Azide, pH 6.5
Purity >95%
Purification Method IMAC followed by desalting
Clonality Monoclonal
Target  
Target di-Ubiquitin (K6-linked)
Species Reactivity Human
Target Function Substrate proteins are linked to ubiquitin via lysine residues on the ubiquitin protein itself (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48 and Lys63). Polyubiquitin chains are formed when a lysine residue of ubiquitin is linked to the carboxyterminal glycine of another ubiquitin. Proteins poly-ubiquinated at specific lysine residues display a tendency to be targeted for different processes ranging from targeting of proteins to degradation in the proteasome, regulated function, subcellular localization, and influencing protein-protein interactions. Due to the limited tools available, the exact function of K6-poly-ubiquitination is largely unknown.
Research Area UPS
Applications

Applications

 

Western Blot

AVA00101 Western Blot with legend
SDS-PAGE & Blotting
Protein samples (200 ng per well) were separated on a Bolt 4-12% Bis-Tris Plus gel (Thermo Fisher) under non-reducing conditions and blotted onto a nitrocellulose membrane using an iBlot2 device (Thermo Fisher)
Blocking
The membrane was blocked using 1 x Casein Block (Sigma) in 1xTBS (1 h, RT, roller)
Wash 1
3 x 5mL of 1xTBS-T (0.05% Tween-20), 5 min, RT, roller
Affimer Incubation
1 in 1000 in 1 x Casein Block (Sigma) in 1xTBS (16 h, 4°C, roller)
Wash 2
3 x 5mL of 1xTBS-T (0.05% Tween-20), 5 min, RT, roller
Detection
1 in 1000 Penta-His HRP (BioRad) in 1 x Casein Block (Sigma) in 1xTBS (1 h, RT°C, roller)
Wash 3
3 x 5mL of 1xTBS-T (0.05% Tween-20), 5 min, RT, roller
Substrate
ECL (Amersham)
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