Anti-diUbiquitin K33 Affimer Reagent – GFP, 7xHis tag

Catalogue CodeAVA00105 Size100μg
 
Anti di-Ubiquitin K33 Affimer® (K33-1) is a superior alternative to K33 antibodies and aptamers.

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Anti-diUbiquitin K33 Affimer Reagent – GFP, 7xHis tag
£200.00
 
Catalogue CodeAVA00105 Size100μg
 
Anti di-Ubiquitin K33 Affimer® (K33-1) is a superior alternative to K33 antibodies and aptamers.
OR
Datasheet

Datasheet

General  
Name Anti-diUbiquitin K33 Affimer Reagent – GFP, 7xHis tag
Catalogue Code AVA00105
Overview  
Clone ID K33-1
Size (μg) 100
Tested Applications Western Blotting
Tags 7X His, GFP
Conjugate Unconjugated
Properties  
Form Liquid
Storage Instructions Store at 4ºC for up to 6 months. For longer term storage, it is recommended to store at -20ºC in single use aliquots.
Buffer 100 mM Sodium Phosphate, 150 mM Sodium Chloride, 0.02% Sodium Azide, pH 7.4
Purity >95%
Purification Method IMAC followed by desalting
Clonality Monoclonal
Target  
Target di-Ubiquitin K33
Species Reactivity Human
Target Function Substrate proteins are linked to ubiquitin via lysine residues on the ubiquitin protein itself (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48 and Lys63). Polyubiquitin chains are formed when a lysine residue of ubiquitin is linked to the carboxy-terminal glycine of another ubiquitin. Proteins poly-ubiquinated at specific lysine residues display a tendency to be targeted for different processes ranging from targeting of proteins to degradation in the proteasome, regulated function, subcellular localization, and influencing protein-protein interactions. Due to the limited tools available, the exact function of K33-poly-ubiquitination is largely unknown.
Research Area UPS
Applications

Applications

 

Western Blot

AVA00105 Western Blot
SDS-PAGE & Blotting
Protein samples (200 ng per well) were separated on a Bolt 4‑12% Bis‑Tris Plus gel (Thermo Fisher) under non-reducing conditions and blotted onto a nitrocellulose membrane using an iBlot2 (Thermo Fisher).
Blocking
The membrane was blocked using 1 x Casein Block (Sigma) in 1 x TBS (1 h, RT, roller)
Wash 1
3 x 5mL of 1 x TBS-T (0.05% Tween-20), 5 min, RT, roller
Affimer Incubation
1 in 1000 in 1 x Casein Block (Sigma) in 1 x TBS (16 h, 4°C, roller)
Wash 2
3 x 5mL of 1 x TBS-T (0.05% Tween-20), 5 min, RT, roller
Detection
1 in 1000 Penta-His HRP (BioRad) in 1 x Casein Block (Sigma) in 1 x TBS (1 h, RT°C, roller)
Wash 3
3 x 5mL of 1 x TBS-T (0.05% Tween-20), 5 min, RT, roller
Substrate
ECL (Amersham)

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