Analytical Ultracentrifugation
Applications include
Determination of solution molecular weight, provides information about shape / denaturation
Quantitative information about molecular mass homogeneity / protein heterogeneity
Detection of binding/aggregation and whether it is a reversible/irreversible process – association constants, aggregation states.
Facilities include
Beckman XL-I Analytical centrifuge ( MW range 102-106 Da, concentration range 0.005 mg/ml – 20 mg/ml, Binding/Dissociation const. range KD of 10-3-10-8 M)